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Generation and immunogenicity of a recombinant pseudorabies virus expressing cap protein of porcine circovirus type 2

Song, Y., Jin, M., Zhang, S., Xu, X., Xiao, S., Cao, S., Chen, H.
Veterinary microbiology 2007 v.119 no.2-4 pp. 97-104
recombinant antigens, Suid alphaherpesvirus 1, protein synthesis, open reading frames, gene expression, vaccine development, genomics, recombinant vaccines, immune response, immunization, Porcine circovirus, lymphocyte proliferation, postweaning multisystemic wasting syndrome, swine
Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS), which is an important economical disease affecting the pig industry worldwide. In order to develop an effective vaccine for PMWS, a recombinant pseudorabies virus (PRV) was generated and tested in piglets in this study. The PCV2 open reading frame 2 (ORF2) gene was inserted into pIECMV plasmid and co-transfected with PRV Tk-/gE-/LacZ+ genome into IBRS-2 cells to generate a recombinant Tk-/gE-/ORF2+ virus. The expression of PCV2 ORF2 gene in the recombinant virus was confirmed by Western blotting and indirect immunofluorescence assay (IFA). Four-week-old piglets were immunized by the recombinant virus, and the immunogenicity of PRV Tk-/gE-/ORF2+ was tested by PRV-enzyme-linked immunosorbent assay (ELISA), PRV neutralizing assay, ORF2-ELISA and ORF2 specific lymphocyte proliferation response. PRV Tk-/gE-/ORF2+ elicited significant humoral immune responses to both PRV and PCV2, and the PCV2-specific lymphocyte proliferation response could be detected on day 49 of this experiment. These findings suggest that the recombinant PRV Tk-/gE-/ORF2+ may be a potential vaccine against both PCV2 and PRV.