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Construction of comparative genetic maps of two 4Bs.4Bl-5Rl translocations in bread wheat (Triticum aestivum L.)

Leach, R.C., Dundas, I.S., Houben, A.
Genome = 2006 v.49 no.7 pp. 729-734
Triticum aestivum, wheat, chromosome mapping, chromosome translocation, nucleic acid hybridization, translocation lines, restriction fragment length polymorphism, genotype, genetic variation, copper, nutrient use efficiency, plant nutrition
The physical length of the rye segment of a 4BS.4BL-5RL translocation derived from the Cornell Wheat Selection 82a1-2-4-7 in a Triticum aestivum 'Chinese Spring' background was measured using genomic in situ hybridization (GISH) and found to be 16% of the long arm. The size of this translocation was similar to previously published GISH measurements of another 4BS.4BL-5RL translocation in a Triticum aestivum 'Viking' wheat background. Molecular maps of both 4BS.4BL-5RL translocations for 2 different wheat backgrounds were developed using RFLP analysis. The locations of the translocation breakpoints of the 2 4BS.4BL-5RL translocations were similar even though they arose in different populations. This suggests a unique property of the region at or near the translocation breakpoint that could be associated with their similarity and spontaneous formation. These segments of rye chromosome 5 also contain a gene for copper efficiency that improves the wheat's ability to cope with low-copper soils. Genetic markers in these maps can also be used to screen for copper efficiency in bread wheat lines derived from the Cornell Wheat Selection 82a1 2-4-7.