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Construction of comparative genetic maps of two 4Bs.4Bl-5Rl translocations in bread wheat (Triticum aestivum L.)

Author:
Leach, R.C., Dundas, I.S., Houben, A.
Source:
Genome = 2006 v.49 no.7 pp. 729-734
ISSN:
0831-2796
Subject:
Triticum aestivum, wheat, chromosome mapping, chromosome translocation, nucleic acid hybridization, translocation lines, restriction fragment length polymorphism, genotype, genetic variation, copper, nutrient use efficiency, plant nutrition
Abstract:
The physical length of the rye segment of a 4BS.4BL-5RL translocation derived from the Cornell Wheat Selection 82a1-2-4-7 in a Triticum aestivum 'Chinese Spring' background was measured using genomic in situ hybridization (GISH) and found to be 16% of the long arm. The size of this translocation was similar to previously published GISH measurements of another 4BS.4BL-5RL translocation in a Triticum aestivum 'Viking' wheat background. Molecular maps of both 4BS.4BL-5RL translocations for 2 different wheat backgrounds were developed using RFLP analysis. The locations of the translocation breakpoints of the 2 4BS.4BL-5RL translocations were similar even though they arose in different populations. This suggests a unique property of the region at or near the translocation breakpoint that could be associated with their similarity and spontaneous formation. These segments of rye chromosome 5 also contain a gene for copper efficiency that improves the wheat's ability to cope with low-copper soils. Genetic markers in these maps can also be used to screen for copper efficiency in bread wheat lines derived from the Cornell Wheat Selection 82a1 2-4-7.
Agid:
703404