PubAg

Main content area

Enzymatic activities of lactic acid bacteria isolated from Cornetto di Matera sourdoughs

Author:
Zotta, T., Ricciardi, A., Parente, E.
Source:
International journal of food microbiology 2007 v.115 no.2 pp. 165-172
ISSN:
0168-1605
Subject:
wheat flour, sourdough, sourdough bread, dough development, isolation, lactic acid bacteria, Leuconostoc mesenteroides subsp. mesenteroides, Lactobacillus curvatus, Lactobacillus plantarum, Lactobacillus pentosus, Weissella cibaria, proteolysis, aminopeptidases, beta-glucosidase, phytases, urease, enzyme activity
Abstract:
Forty-one strains of lactic acid bacteria (LAB) isolated from Cornetto di Matera sourdoughs were screened for their enzymatic activities, to elucidate their possible roles during the fermentation process. Urease, peptidase, phytase, phosphatase and β-glucosidase activities were measured spectrophotometrically using synthetic substrates. Proteolytic activities were examined in model doughs, using neutral and acidified sterile doughs as controls. All strains had low urease, glutamyl aminopeptidase and iminopeptidase activities, whereas differences within species were observed for the other enzymatic activities. Leuconostoc mesenteroides and Lactobacillus curvatus strains generally showed high aminopeptidase, X-prolyl dipeptidyl aminopeptidase, β-glucosidase and phytase activities, while the enzymatic activities of Lactobacillus plantarum, Lactobacillus pentosus and Weissella cibaria varied between strains. In order to classify the strains on the basis of similar enzymatic profiles, a hierarchical cluster analysis was carried out. Several strains of L. plantarum, L. curvatus and Leuc. mesenteroides showed an interesting combination of proteolytic, peptidase, β-glucosidase and phytase activities, suggesting their possible usefulness as a mixed starter culture in bread-making processes.
Agid:
704861