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Inhibition of Salmonella binding to porcine intestinal cells by a wood-derived prebiotic

Božic Aleksandar, Anderson Robin C., Crippen Tawni L., Swaggerty Christina L., Hume Michael E., Beier Ross C., He Louis Haiqi, Genovese Kenneth J., Poole Toni L., Harvey Roger B., Nisbet David James
Microorganisms v.8 no.7 pp. 1051
Saccharomyces cerevisiae var. cerevisiae, Salmonella Typhimurium, antibiotic resistance, bacterial adhesion, binding properties, enteropathogenic Escherichia coli, enteropathogens, epithelial cells, galactose, glucose, host cell invasion, intestinal mucosa, mannose, oligosaccharides, prebiotics, swine, wood fibers, xylose
Numerous Salmonella enterica serovars can cause disease and contamination of animal-produced foods. Antimicrobial resistance in these as well as other bacteria in animal agriculture may render antibiotics ineffective and provides motivation to develop non-antibiotic strategies to control animal diseases. Oligosaccharide-rich products capable of blocking pathogen adherence to the animal's intestinal mucosa are attractive alternatives to antibiotics as these have potential to prevent enteric infections. Presently, a wood-derived prebiotic composed mainly of glucose-galactose-mannose-xylose oligomers was found to inhibit mannose-sensitive binding of select Salmonella enterica serovar Typhimurium and enteropathogenic Escherichia coli strains when reacted with Saccharomyces boulardii. Tests for the ability of the prebiotic to prevent binding of a green fluorescent protein (GFP)-labeled Salmonella Typhimurium to intestinal porcine epithelial cells (IPEC-J2) cultured in vitro revealed that prebiotic-exposed GFP-labeled S. Typhimurium suspensions bound 32 to 34% fewer (P < 0.05) individual IPEC-J2 cells than did GFP-labeled S. Typhimurium suspensions having no prebiotic exposure. Quantitatively, 90% fewer (P < 0.05) prebiotic-exposed GFP-labeled S. Typhimurium cells were bound per individual IPEC-J2 cell compared to counts obtained for non-prebiotic exposed GFP-labeled S. Typhimurium, the latter, depending on challenge level, averaging 0.26 to 13.77 GFP-labeled S. Typhimurium cells/IPEC-J2 cell, respectively. Comparison of invasiveness of S. Typhimurium DT104 against IPEC-J2 cells revealed greater than 90% decrease (P < 0.05) in intracellular recovery of prebiotic-exposed S. Typhimurium DT104 when compared to non-exposed controls (averaging 4.4 ± 0.2 Log10 CFU/well). These results suggest compounds within the wood-derived prebiotic bound to E. coli- and S. Typhimurium-produced adhesions and in the case of S. Typhimurium, this adhesion-binding activity inhibited the binding and invasion of IPEC-J2 cells.