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Identification of Sex Pheromone Components of the Box Tree Pyralid, Glyphodes perspectalis

Kawazu, Kei, Honda, Hiroshi, Nakamura, Suguru, Adati, Tarô
Journal of chemical ecology 2007 v.33 no.10 pp. 1978-1985
Pyralidae, sex pheromones, chemical composition, aldehydes, pheromone blends, ovipositor, antennae, electrophysiology, electroantennography, sex attractants, pest monitoring, Buxus microphylla, Japan
Analysis of ovipositor extracts of the box tree pyralid, Glyphodes perspectalis (Lepidoptera: Crambidae), by coupled gas chromatography-electroantennogram detection and gas chromatography yielded three candidate pheromone compounds. Gas chromatography-mass spectrometry, and reaction with dimethyl disulfide (DMDS), identified these compounds as (Z)-11-hexadecenal (Z11-16:Ald), (E)-11-hexadecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z11-16:OH). The ratio of these three compounds in crude extract was 5:1.25:1, and the total amount was approximately 100 ng/female. In laboratory bioassays, Z11-16:Ald alone was attractive to males. In field bioassays, however, Z11-16:Ald alone was not attractive to males, but a mixture of Z11-16:Ald and E11-16:Ald was. The addition of Z11-16:OH to the two aldehydes, significantly reduced attractiveness to male moths in a field trial. From these results, we conclude that the aldehyde compounds comprise the sex pheromone components of G. perspectalis. A rubber septum containing 0.6 mg of the two aldehydes at the natural ratio was an effective lure for monitoring this pest.