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Effects of dietary fish oil on lipid peroxidation and serum triacylglycerol levels in psychologically stressed mice

Author:
Oarada, M., Tsuzuki, T., Gonoi, T., Igarashi, M., Kamei, K., Nikawa, T., Hirasaka, K., Ogawa, T., Miyazawa, T., Nakagawa, K.
Source:
Nutrition 2008 v.24 no.1 pp. 67-75
ISSN:
0899-9007
Subject:
mice, animal models, animal stress, dietary supplements, fish oils, lipid peroxidation, blood lipids, triacylglycerols, distress, omega-3 fatty acids, polyunsaturated fatty acids, soybean oil, olive oil, thiobarbituric acid-reactive substances, liver, kidneys, antioxidant activity
Abstract:
Objective: The intake of ω-3 polyunsaturated fatty acids and psychological stress can each induce tissue lipid peroxidation. In our present study, we investigated their combined effects on the oxidative status of mouse tissues. Methods: Mice were group-housed (four mice/cage) and fed a diet containing fish oil (as a source of ω-3 polyunsaturated fatty acids), soybean oil, or olive oil for 3 wk. These animals were then 1) housed under the same conditions (four per cage, control group) or 2) individually housed to generate psychological stress conditions (isolation stress). After 2 wk of isolation stress, the levels of thiobarbituric acid-reactive substances (an index of lipid peroxidation) and antioxidants in the liver and kidney and the serum levels of triacylglycerol were measured. Results: Fish oil-fed mice showed increased levels of thiobarbituric acid-reactive substances in their livers and kidneys compared with soybean oil- or olive oil-fed mice. These increases in thiobarbituric acid-reactive substance levels in the fish oil-fed mice were less profound under isolation stress conditions when compared with the group-housed animals on the same diet. In the fish oil-fed mice, isolation stress led to an increase in liver vitamin E levels when compared with their group-housed counterparts. The fish oil-fed mice exhibited lower serum triacylglycerol levels compared with the soybean oil- or olive oil-fed mice, and this decrease was more profound under conditions of isolation stress when compared with group-housing conditions. Conclusion: Dietary fish oil combined with isolation stress results in lower levels of lipid peroxidation in the liver and kidney compared with dietary fish oil alone.
Agid:
717845