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In Vivo Anti-influenza Virus Activity of an Immunomodulatory Acidic Polysaccharide Isolated from Cordyceps militaris Grown on Germinated Soybeans

Ohta, Y., Lee, J.B., Hayashi, K., Fujita, A., Park, D.K., Havashi, T.
Journal of agricultural and food chemistry 2007 v.55 no.25 pp. 10194-10199
Cordyceps, microbial growth, soybeans, seed germination, polysaccharides, carbohydrate composition, antiviral properties, Influenza A virus, interleukins, viral diseases of animals and humans, tumor necrosis factor-alpha, nitric oxide, nitric oxide synthase, gene expression, messenger RNA, macrophages, mice, interferons
An acidic polysaccharide (APS) was isolated from the extract of Cordyceps militaris grown on germinated soybeans. Analyses of sugar composition indicated that APS consisted of D-galactose, L-arabinose, D-xylose, L-rhamnose, and D-galacturonic acid. On the basis of the result of methylation analysis, APS was considered to be mainly composed of Araf-(1 leads to , leads to 5)-Araf-(1 leads to, leads to 4)-Galp-(1 leads to and leads to 4)-GalAp-(1 leads to residues. When the polysaccharide was intranasally administered, it decreased virus titers in the bronchoalveolar lavage fluid and the lung of mice infected with influenza A virus and increased survival rate. Furthermore, APS increased TNF-α and IFN-γ levels in mice when compared with those of untreated mice. APS enhanced nitric oxide (NO) production and induced iNOS mRNA and protein expressions in RAW 264.7 murine macrophage cells. The induction of mRNA expression of cytokines including IL-1β, IL-6, IL-10, and TNF-α was also observed. These results demonstrated that APS might have beneficial therapeutic effects on influenza A virus infection at least in part by modulation of the immune function of macrophages.