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Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues

Okui, Y., Kano, R., Maruyama, H., Hasegawa, A.
Veterinary immunology and immunopathology 2008 v.121 no.1-2 pp. 156-160
interspecific variation, messenger RNA, complementary DNA, physiological regulation, normal values, dogs, reverse transcriptase polymerase chain reaction, amino acid sequences, gene expression, animal organs, genes, immune response, immune system, molecular cloning, receptors, nucleotide sequences, animal tissues, interferons
Toll-like receptor 7 (TLR7) is activated by single strand RNA and imidazoquinoline compounds, and induces interferon production. In this study, canine TLR7 cDNA was cloned and sequenced. The full-length cDNA of canine TLR7 gene was 3419 bp, encoding 1032 amino acids. The similarities of canine TLR7 with human and mouse TLR7 were 84 and 80% at the nucleotide sequence level, and 86 and 79% at amino acid sequence level, respectively. Further, the expression of TLR7 mRNA was investigated in canine normal tissues by semiquantitative RT-PCR analysis. The common expression level of TLR7 mRNA in tissues from three dogs examined was in large intestine, lung, pancreas, small intestine and skin, though the expression level in each tissue was varied among these healthy dogs. In other tissues (kidney, liver, lymph node, spleen, adrenal gland, and PBMCs), the level of TLR7 mRNA expression was different in individuals.