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Pulmonary cryptococcosis in slaughtered sheep: Anatomopathology and culture

Lemos, L.S., Santos, A.S. de O. dos, Vieira-da-Motta, O., Texeira, G.N., Carvalho, E.C.Q. de
Veterinary microbiology 2007 v.125 no.3-4 pp. 350-354
sheep, sheep diseases, respiratory tract diseases, mycoses, Cryptococcus neoformans, disease detection, case studies, epidemiological studies, pathogen identification, lungs, tissue analysis, cryopreservation, histopathology, yeasts, epithelium, viability, microscopy, differential staining, in vitro culture, risk assessment, disease transmission, zoonoses, Brazil
Cryptococcosis is considered a zoonosis but is rare in stock animals. In humans it is imputed to be a mortality cause in immunocompromised patients. This case study reports the anatomopathology of respiratory cryptococcosis in ovine and the recuperation and identification of Cryptococcus neoformans var. neoformans from 1-year frozen lung tissue sample. The findings from haematoxylin/eosin staining microscopy and special staining showed the destruction of the columnar respiratory epithelium architecture associated to suppuration, and the growth of spherical yeast buddings sometimes involved by clear halo produced by unstained capsular substance. The agent was identified by miniApi ID32C, while variety neoformans was further identified by culture in CGB agar. The diagnosis in stock animal suggests the involvement of an exogenous source of the agent as an alert to public health and contributes for epidemiological investigations.