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Application of preparative high-speed counter-current chromatography for separation and purification of lignans from Taraxacum mongolicum

Author:
Shi, Shuyun, Zhang, Yuping, Huang, Kelong, Liu, Suqin, Zhao, Yu
Source:
Food chemistry 2008 v.108 no.1 pp. 402-406
ISSN:
0308-8146
Subject:
countercurrent chromatography, separation, purification, lignans, Taraxacum, plant extracts, phytochemicals, chemical analysis
Abstract:
Apart from being used as a pharmaceutical, the inflorescences, leaves and roots of Taraxacum mongolicum are processed into different food products. However, only few phytochemical investigations on this plant have been performed. In the present study, a preparative high-speed counter-current chromatography (HSCCC) for the separation and purification of bioactive compounds from T. mongolicum was developed. Two lignans, mongolicumin A and rufescidride, were obtained. The target compounds were finally isolated and purified with a solvent system composed of ethyl acetate-n-butanol-water (2:5:7, v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. By injecting 500mg of the enriched extract of T. mongolicum, one-step HSCCC procedure yielded 36.7mg of mongolicumin A and 43.9mg of rufescidride with the purity of 98.7% and 98.5%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of the two lignans were confirmed by UV, IR, HRESIMS, 1D and 2D NMR. Among them, mongolicumin A is a new compound, and rufescidride was obtained from genus Taraxacum for the first time. Furthermore, lignans were first isolated and identified from genus Taraxacum.
Agid:
722214