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Dietary administration of sodium alginate and κ-carrageenan enhances the innate immune response of brown-marbled grouper Epinephelus fuscoguttatus and its resistance against Vibrio alginolyticus

Author:
Cheng, Ann-Chang, Chen, Yu-Yuan, Chen, Jiann-Chu
Source:
Veterinary immunology and immunopathology 2008 v.121 no.3-4 pp. 206-215
ISSN:
0165-2427
Subject:
grouper, Epinephelus, animal nutrition, dietary supplements, alginates, carrageenan, physiological response, animal health, health status, immune system, disease resistance, bacterial infections, Vibrio alginolyticus, immune response, cell-mediated immunity, humoral immunity, phagocytosis, complement, enzyme activity, lysozyme, protective effect
Abstract:
Brown-marbled grouper Epinephelus fuscoguttatus which had been fed diets containing sodium alginate and kappa (κ)-carrageenan at 5, 10 and 20gkg⁻¹, respectively after 0, 2, 4, 6, 8 and 14 weeks were examined for survival, growth, innate cellular and humoral responses as compared to the fish that fed control non-supplemented diet. Survival was 100% for the fish that fed all diets after 14 weeks and no significant difference in growth was observed among seven diets. The fish that fed a diet containing sodium alginate at 10gkg⁻¹, and fed a diet containing κ-carrageenan at 5gkg⁻¹ over 2-8 weeks showed significantly increased leucocyte count, respiratory burst, phagocytic activity and phagocytic index, The fish that fed a diet containing sodium alginate at 5gkg⁻¹, and fed a diet containing κ-carrageenan at 5gkg⁻¹ over 2-8 weeks showed significantly increased alternative complement activity (ACH50) and lysozyme activity. In another experiment, E. fuscoguttatus, which had been fed control diet, and all diets containing sodium alginate, and κ-carrageenan after 14 weeks were challenged with Vibrio alginolyticus at 5.0x10⁹ colony-forming units (cfu) fish⁻¹ and then placed in seawater of 34[per thousand]. The survival of fish that fed a diet containing sodium alginate at 10gkg⁻¹ and κ-carrageenan at 5gkg⁻¹ was significantly higher than that of fish which fed the control diet over 96-168h. It was concluded that E. fuscoguttatus which fed a diet containing sodium alginate at 10gkg⁻¹ or less, or fed a diet containing κ-carrageenan at 5gkg⁻¹ enhance the innate immunity, and increase the resistance from V. alginolyticus infection.
Agid:
722935