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Reproduction traits of heterozygous myostatin knockout sows crossbred with homozygous myostatin knockout boars

Author:
Sheng‐zhong Han, Zhou‐Yan Li, Hyo‐Jin Paek, Hak‐Myong Choe, Xi‐Jun Yin, Biao‐Hu Quan
Source:
Reproduction in domestic animals 2021 v.56 no.1 pp. 26-33
ISSN:
0936-6768
Subject:
boars, dystocia, genes, genotyping, heterozygosity, homozygosity, lean meat, litter size, mutants, mutation, myostatin, phenotype, pregnancy, reproductive performance, semen quality
Abstract:
Few studies exist on homozygous myostatin gene mutant (MSTN⁻/⁻) pigs, especially on their reproductive ability. We have previously shown that semen quality of homozygous MSTN⁻/⁻ boars is comparable to that of wild type (WT). However, no data exist on the reproductive ability of heterozygous MSTN gene mutant (MSTN⁺/⁻) sows. The present study highlights showed that the heterozygous MSTN⁺/⁻ sows have delayed pubertal age than WT sows (255.80 ± 6.79 versus 191.10 ± 3.42, respectively). The number of services per pregnancy of heterozygous MSTN⁺/⁻ sows is significantly higher than that of WT sows (3.33 ± 0.43 versus 1.60 ± 0.25, respectively). Moreover, although heterozygous MSTN⁺/⁻ sows have natural reproduction ability, their litter size was significantly lower than that of WT sows (7.75 ± 0.44 versus 14.25 ± 0.60, respectively). Offsprings generated from heterozygous MSTN⁺/⁻ sow and homozygous MSTN⁻/⁻ boar were genotyped with the PCR and sequencing method to detect myostatin mutation and to identify whether the piglets are homozygous MSTN⁻/⁻ or heterozygous MSTN⁺/⁻. The proportion of homozygous MSTN⁻/⁻ piglets was significantly lower than that of heterozygous MSTN⁺/⁻ piglets (2.50 ± 0.35 versus 5.25 ± 0.60, respectively). Furthermore, none of the sows presented dystocia, and the phenotype of heterozygous MSTN⁺/⁻ piglets was normal. However, 10% homozygous MSTN⁻/⁻ piglets died of dyspnoea within 2 hr after birth, 60% of homozygous MSTN⁻/⁻ piglets showed large tongues, and 50% had umbilical hernias. In summary, this study for the first time reports the reproduction traits of heterozygous MSTN⁺/⁻ sows crossbred with homozygous MSTN⁻/⁻ boars. This study will pave the way in a new direction for the breeding and development of super lean meat varieties in the future.
Agid:
7239525