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Effect of agitation on heat-induced deproteination process of buffalo milk whey

Agrawal, Shailesh G., Bund, Rajesh K., Pandit, Aniruddha B.
Journal of food engineering 2008 v.87 no.3 pp. 398-404
buffalo milk, whey, heat treatment, agitation, continuous systems, calcium chloride, protein aggregates, denaturation, kinetics, shear strength, shear stress, particle size distribution, protein content, lactose, pH, polyacrylamide gel electrophoresis
The heat induced deprotienation of buffalo milk whey was carried out in a bench top continuous process after optimization of process parameters for maximum protein removal at lab scale. The optimum conditions for maximum heat induced aggregation were found to be 92±2°C, 30min at a pH 5.3±0.1 and in the presence of 10mM CaCl₂. The CSTR (continuous stirred tank reactor) was then used as a tool to study the kinetics of heat induced denaturation at different agitation rates. The kinetics was also studied with batch system under similar conditions. The values of deproteination rate constants were in close agreement from both batch and continuous studies. The shear rate was found to have great effect on the protein denaturation kinetics as well as protein aggregate size distribution. The rate of deproteination was found to increase as the shear rate increased. The denaturation kinetics also changed from second order to first order as the shear was increased from 360s⁻¹ to 1020s⁻¹, in both batch as well as continuous process.