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Profiling gibberellin (GA₃)-responsive genes in mature mandarin fruit using a citrus 22K oligoarray

Author:
Fujii, Hiroshi, Shimada, Takehiko, Sugiyama, Aiko, Endo, Tomoko, Nishikawa, Fumie, Nakano, Michiharu, Ikoma, Yoshinori, Shimizu, Tokuro, Omura, Mitsuo
Source:
Scientia horticulturae 2008 v.116 no.3 pp. 291-298
ISSN:
0304-4238
Subject:
Citrus unshiu, mandarins, gibberellins, microarray technology, gene expression, gene expression regulation, genes, transcription (genetics), pathogenesis-related proteins, photosynthesis, chloroplasts, plant proteins, resistance mechanisms, plant stress, ethylene, ripening
Abstract:
Gibberellin₃ (GA₃)-responsive genes were investigated with a citrus 22K oligoarray to further the understanding of transcriptional regulation by GA₃ treatment in Satsuma mandarin fruit (Citrus unshiu Marc.). 213 GA₃-responsive genes were identified that showed a 3-fold or greater expression change after 72h GA₃ treatment, compared to expression after 72h air treatment. GA₃ treatment induced expression of pathogenesis-related (PR) proteins and genes that function in photosynthesis, chloroplast biogenesis, resistance, defense and stress. Also, GA₃ treatment reduced the transcription of several ethylene-inducible genes, such as carotenoid metabolic genes, which are associated with fruit ripening. Contrasting effects between GA₃ and ethylene were observed on photosynthesis and chloroplast biogenesis, chlorophyll metabolism, and carotenoid metabolism, indicating that the endogenous GA₃ level might be important for the endogenous regulation of maturation and senescence in mature citrus fruit. It was also found that the GA response pathway was likely to take part in cross-talk with the pathogen-related pathway in mature citrus fruit.
Agid:
727278