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Production of extracellular bifidogenic growth stimulator (BGS) from Propionibacterium shermanii using a bioreactor system with a microfiltration module and an on-line controller for lactic acid concentration
- Kouya, Tomoaki, Tobita, Kazuhiro, Horiuchi, Masahito, Nakayama, Eri, Deguchi, Hiroyoshi, Tanaka, Takaaki, Taniguchi, Masayuki
- Journal of bioscience and bioengineering 2008 v.105 no.3 pp. 184-191
- Propionibacterium freudenreichii subsp. shermanii, cell culture, bioreactors, culture media, lactic acid, continuous systems, microfiltration, controllers
- Production of a bifidogenic growth stimulator (BGS) by Propionibacterium freudenreichii subsp. shermanii (Propionibacterium shermanii) using lactic acid as a carbon source was investigated using different cultivation methods. When a continuous bioreactor system with a filtration device was used at a dilution rate of 0.075 h⁻¹, the average BGS concentration was 2.4 mg/l, which corresponds to a BGS productivity per cultivation time of 1.8x10⁻¹ mg·l ⁻¹·h⁻¹. The BGS productivity per cultivation time in continuous cultivation with filtration was 1.9-fold that (9.4x10⁻² mg·l ⁻¹·h⁻¹) in a conventional batch cultivation. In fed-batch cultivation with feed-back control using an on-line lactic acid controller with a lactic acid biosensor, it was possible to prevent substrate inhibition by maintaining the lactic acid concentration in culture broth low at 3.3 g/l, and an enhanced BGS production (31 mg/l) was successfully attained. The BGS productivity per cultivation time (2.1x10⁻¹ mg·l ⁻¹·h⁻¹) in the fed-batch cultivation with feed-back control was 2.2-fold that in the conventional batch cultivation. A new bioreactor system was developed by coupling a continuous bioreactor system with a filtration device to an on-line lactic acid controller. Using the new bioreactor system, we produced BGS continuously at a high level of 47 mg/l. The BGS productivities per cultivation time (3.5 mg·l ⁻¹·h⁻¹) and the total volume of medium used (1.7x10⁻¹ mg·l ⁻¹·h⁻¹) obtained in the new bioreactor system were 37-fold and 2.1-fold those in the conventional batch cultivation, respectively. These results described above clearly demonstrate the positive effects of both the continuous filtration for removal of metabolites (propionic and acetic acids) inhibitory to cell growth and feed-back control of lactic acid concentration in the culture broth on BGS production by P. shermanii. This paper is the first report on BGS production by the propionic acid bacterium using lactic acid as a carbon source.