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Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP⁺-dependent xylitol dehydrogenase, and xylulokinase
- Matsushika, Akinori, Watanabe, Seiya, Kodaki, Tsutomu, Makino, Keisuke, Sawayama, Shigeki
- Journal of bioscience and bioengineering 2008 v.105 no.3 pp. 296-299
- Saccharomyces cerevisiae, genetically engineered microorganisms, gene expression, alcohol oxidoreductases, kinases, xylose, carbohydrate metabolism, alcoholic fermentation, ethanol production, ethanol, genetic transformation, gene transfer, genes, Pichia stipitis
- We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP⁺-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.