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Preparative separation of four triterpene saponins from radix astragali by high-speed counter-current chromatography coupled with evaporative light scattering detection
- Peng, Jinyong, Dong, Fuqiu, Qi, Yan, Han, Xu, Xu, Youwei, Xu, Lina, Xu, Qiwei, Liu, Kexin, Zhu, Zhenyu
- Phytochemical analysis 2008 v.19 no.3 pp. 212-217
- Astragalus membranaceus, medicinal plants, phytochemicals, triterpenoid saponins, chemical analysis, chemical structure, spectral analysis, countercurrent chromatography, solvents, purity
- Four triterpene saponins, including astragaloside IV, astragaloside II, astragaloside I and acetylastragaloside I, were successfully isolated and separated by high-speed counter-current chromatography coupled with evaporative light scattering detection from Radix Astragali using stepwise elution with a pair of solvent systems composed of n-hexane:ethyl acetate:ethanol:water in volume ratios of 1:0.6:0.6:1 and 1:1:1:1 (by volume). The isolation produced 26.5 mg astragaloside IV, 28.2 mg astragaloside II, 48.7 mg astragaloside I and 17.6 mg acetylastragaloside I with purities of 97.6, 96.4, 98.8 and 96.8%, respectively, determined by high-performance liquid chromatography from 250 mg crude extract. The chemical structures of the isolated compounds were identified by UV, NMR and MS, and confirmed by authentic standards.