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Artificial intelligence-assisted identification and quantification of osteoclasts

Author:
Thomas Emmanuel, Annemarie Brüel, Jesper Skovhus Thomsen, Torben Steiniche, Mikkel Bo Brent
Source:
MethodsX 2021 v.8 pp. 101272
ISSN:
2215-0161
Subject:
artificial intelligence, bone resorption, cathepsin K, color, computer software, microscopy, osteoclasts
Abstract:
Quantification of osteoclasts to assess bone resorption is a time-consuming and tedious process. Since the inception of bone histomorphometry and manual counting of osteoclasts using bright-field microscopy, several approaches have been proposed to accelerate the counting process using both free and commercially available software. However, most of the present alternatives depend on manual or semi-automatic color segmentation and do not take advantage of artificial intelligence (AI). The present study directly compare estimates of osteoclast-covered surfaces (Oc.S/BS) obtained by the conventional manual method using a bright-field microscope to that obtained by a new AI-assisted method. We present a detailed step-by-step guide for the AI-based method. Tibiae from Wistar rats were either enzymatically stained for TRAP or immunostained for cathepsin K to identify osteoclasts. We found that estimation of Oc.S/BS by the new AI-assisted method was considerably less time-consuming, while still providing similar results to the conventional manual method. In addition, the retrainable AI-module used in the present study allows for fully automated overnight batch processing of multiple annotated sections.•Bone histomorphometry•AI-assisted osteoclast identification•TRAP and cathepsin K
Agid:
7285067