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A novel branched αβ-glucan isolated from the basidiocarps of the edible mushroom Pleurotus florida
- Santos-Neves, J.C., Pereira, M.I., Carbonero, E.R., Gracher, A.H.P., Alquini, G., Gorin, P.A.J., Sassaki, G.L., Iacomini, M.
- Carbohydrate polymers 2008 v.73 no.2 pp. 309-314
- Pleurotus florida, chemical composition, mushrooms, glucans, extraction, extracts, fruiting bodies, chemical structure
- As polysaccharides of the mushroom genus Pleurotus can act as biological response modifiers, we now describe the chemical characterization of a new water-soluble αβ-glucan from the fruiting bodies of the edible mushroom Pleurotus florida. It was obtained via successive aq. extraction, freeze-thawing, precipitation with Fehling solution, and dialysis through a membrane with M(r) 1000 kDa cut-off. It was analyzed using NMR spectroscopy, HPSEC, monosaccharide composition, methylation analysis, and a controlled Smith degradation. It was a branched glucan (M(w) 1.1 x 10(6) g/mol), with a main-chain of (1 leads to 3)-linked-α-D-Glcp residues, partially substituted at O-6 by single-unit β-D-Glcp, and a minor proportion by side-chains with 3-O-substituted β-D-Glcp groups.