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Anti-inflammation activity of fruit essential oil from Cinnamomum insularimontanum Hayata
- Lin, Chien-Tsong, Chen, Chi-Jung, Lin, Ting-Yu, Tung, Judia Chen, Wang, Sheng-Yang
- Bioresource technology 2008 v.99 no.18 pp. 8783-8787
- Cinnamomum, medicinal plants, medicinal properties, fruits (plant anatomy), essential oils, anti-inflammatory activity, gas chromatography, mass spectrometry, chemical composition, nitric oxide, bioassays, citral, inhibitory concentration 50, protein synthesis, dosage, dose response, biochemical pathways, mechanism of action, enzyme activity
- In this study, the fruit essential oil of Cinnamomum insularimontanum was prepared by using water distillation. Followed by GC-MS analysis, the composition of fruit essential oil was characterized. The main constituents of essential oil were α-pinene (9.45%), camphene (1.70%), β-pinene (4.30%), limonene (1.76%), citronellal (24.64%), citronellol (16.78%), and citral (35.89%). According to the results obtained from nitric oxide (NO) inhibitory activity assay, crude essential oil and its dominant compound (citral) presented the significant NO production inhibitory activity, IC₅₀ of crude essential oil and citral were 18.68 and 13.18μg/mL, respectively. Moreover, based on the results obtained from the protein expression assay, the expression of IKK, iNOS, and nuclear NF-κB was decreased and IκBα was increased in dose-dependent manners, it proved that the anti-inflammatory mechanism of citral was blocked via the NF-κB pathway, but it could not efficiently suppress the activity on COX-2. In addition, citral exhibited a potent anti-inflammatory activity in the assay of croton oil-induced mice ear edema, when the dosage was 0.1 and 0.3mg per ear, the inflammation would reduce to 22% and 83%, respectively. The results presented that the fruit essential oil of C. insularimontanum and/or citral may have a great potential to develop the anti-inflammatory medicine in the future.