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A simple method for bakers' yeast cell disruption using a three-phase fluidized bed equipped with an agitator

Charinpanitkul, Tawatchai, Soottitantawat, Apinan, Tanthapanichakoon, Wiwut
Bioresource technology 2008 v.99 no.18 pp. 8935-8939
Saccharomyces cerevisiae, bakers yeast, inactivation, fluidized beds, agitation, cytolysis, validity, crude protein, solubility, grinding, shear stress
A three-phase fluidized bed equipped with a turbine agitator was utilized as a simple device for disrupting bakers' yeast cells (Saccharomyces cerevisiae). The degree of yeast cell disruption was evaluated based on the number of broken cells and its validity was confirmed by the total amount of crude soluble proteins released and by microscopic observation. It was found that the equipment could yield 90% of yeast cell disruption. With the presence of glass beads, the degree of cell disruption became higher as agitating speed is increased. The disruption enhancement would be attributed to the grinding effect resulting from the interaction between yeast cells and glass beads. One-thousand micrometers of glass beads yielded a higher degree of disruption than larger ones. An increase in liquid flow rate hindered the degree of disruption because of shorter contact time although the shear rates in the yeast suspension would become more rigorous.