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Structural and functional homology among chicken, duck, goose, turkey and pigeon interleukin-8 proteins
- Wu, Yung Fu, Shien, Jui Huang, Yin, Hsien Hseng, Chiow, Shiou Her, Lee, Long Huw
- Veterinary immunology and immunopathology 2008 v.125 no.3-4 pp. 205-215
- chickens, ducks, geese, turkeys, animal proteins, immune system, interleukin-8, immunogenetics, genetic variation, antigenic variation, phylogeny, molecular genetics, molecular cloning, nucleotide sequences, amino acid sequences, sequence homology, chemotaxis
- Interleukin (IL)-8-encoding regions of five avian species were cloned, sequenced and characterized. Each IL-8-encoding region is 312 nucleotides long and encodes IL-8 which is 103 amino acids. Pairwise sequence analysis showed that sequence identities of IL-8-encoding regions ranged from 87% to 100%. The IL-8 protein identities varied from 84% to 100%. Phylogenetic analysis indicated that IL-8-encoding regions and encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from binding reactivities of antiserum against each recombinant IL-8 (rIL-8) protein to homologous or heterologous rIL-8 proteins, chemotactic activities of each rIL-8 protein or reduction levels of the chemotactic activity of rIL-8 protein which was pretreated with homologous or heterlogous antiserum have suggested that all five IL-8 proteins were functionally active, and shared structural and functional identity with each other.