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Effect of plasmid DNA encoding the porcine granulocyte-macrophage colony-stimulating factor on antigen-presenting cells in pigs

Author:
Melkebeek, V., Van den Broeck, W., Verdonck, F., Goddeeris, B.M., Cox, E.
Source:
Veterinary immunology and immunopathology 2008 v.125 no.3-4 pp. 354-360
ISSN:
0165-2427
Subject:
swine, swine diseases, infectious diseases, disease prevention, vaccination, pretreatment, vaccine adjuvants, granulocyte-macrophage colony-stimulating factor, injection, skin, plasmids, immune response, injection site, epithelium, antigen-presenting cells, dendritic cells, Langerhans cells, lymph nodes, immunostimulants
Abstract:
We previously demonstrated that intradermal (ID) delivery of plasmid DNA encoding the porcine granulocyte-macrophage colony-stimulating factor (GM-CSF) 7 days before DNA vaccination enhances both cellular and humoral responses in pigs. In the present work, we studied the effect of the GM-CSF gene on antigen-presenting cells (APC) in pigs. We demonstrated that ID delivery of this gene significantly increased the number of epidermal CD1⁺ cells (Langerhans' cells, skin dendritic cells) at the injection site at day 7. This was accompanied by an enhanced percentage of APC at the immune induction site following DNA vaccination, whereas a positive effect on APC maturation could not be demonstrated. Taken together, our data suggest that both DC recruitment to the immunization site and expansion of APC in the draining LN following DNA vaccination might contribute to the immune enhancing effect of plasmid encoded GM-CSF in pigs.
Agid:
738088