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A 3-O-methylated mannogalactan from Pleurotus pulmonarius: Structure and antinociceptive effect

Smiderle, F.R., Olsen, L.M., Marcon, R., Baggio, C.H., Freitas, C.S., Santos, A.R.S., Torri, G., Gorin, P.A.J., Iacomini, M.
Phytochemistry 2008 v.69 no.15 pp. 2731-2736
anti-inflammatory activity, galactans, Pleurotus pulmonarius, muscle contraction, monosaccharides, methylation, smooth muscle, human health, mice, mushrooms, chemical structure, analgesic effect
A polysaccharide (M(w) 2.39 x 10⁴ g/mol) was extracted with cold water from the basidiomycete Pleurotus pulmonarius, and its antinociceptive and anti-inflammatory properties were evaluated. It was a mannogalactan (MG), whose structure was characterized using mono- and two-dimensional NMR spectroscopy, methylation analysis, and a controlled Smith degradation. It had a main chain of (1 to 6)-linked α-d-galactopyranosyl and 3-O-methyl-α-d-galactopyranosyl units, both of which are partially substituted at O-2 by β-d-mannopyranosyl non-reducing ends. The MG was tested for its effects on the acetic acid-induced writhing reaction in mice, a typical model for inflammatory pain, causing a marked and dose-dependent inhibition of the nociceptive response, with ID₅₀ of 16.2 (14.7-17.7) mg/kg and inhibition of 93 +/- 3% at a dose of 30 mg/kg. An inflammatory response was not inhibited.