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Analgesic and anti-inflammatory effects of the dry matter of culture broth of Termitomyces albuminosus and its extracts
- Lu, Yi-Yu, Ao, Zong-Hua, Lu, Zhen-Ming, Xu, Hong-Yu, Zhang, Xiao-Mei, Dou, Wen-Fang, Xu, Zheng-Hong
- Journal of ethnopharmacology 2008 v.120 no.3 pp. 432-436
- Oriental traditional medicine, dry matter content, culture media, analgesic effect, anti-inflammatory activity, Termitomyces, plant extracts, saponins, polysaccharides, animal disease models, ear diseases, edema, chemical constituents of plants, China
- Aim of the study: The objectives of this study were to investigate the analgesic and anti-inflammatory effects of the dry matter of culture broth (DMCB) of Termitomyces albuminosus in submerged culture and its crude saponin extract (CSE) and crude polysaccharide extract (CPE). Materials and methods: The analgesic effects of DMCB, CSE and CPE were evaluated with models of acetic acid-induced writhing response and formalin test in mouse. The anti-inflammatory effects of DMCB, CSE and CPE were evaluated by using models of xylene-induced mouse ear swelling and carrageen-induced mouse paw edema. Results: The DMCB, CSE and CPE significantly decreased the acetic acid-induced writhing response and the licking time on the late phase in the formalin test. Treatment of DMCB (1000mg/kg), CSE (200mg/kg) or CPE (200mg/kg) inhibited the mouse ear swelling by 61.8%, 79.0% and 81.6%, respectively. In the carrageen-induced mouse paw edema test, the group treated with indomethacin showed the strongest inhibition of edema formation by 77.8% in the third hour after carrageenan administration, while DMCB (1000mg/kg), CSE (200mg/kg) and CPE (200mg/kg) showed 48.4%, 55.6% and 40.5%, respectively. Conclusions: The results suggested that DMCB of Termitomyces albuminosus possessed the analgesic and anti-inflammatory activities. Saponins and polysaccharides were proposed to be the major active constituents of Termitomyces albuminosus in submerged culture.