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Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses in RAW264.7 macrophages

Hu, Xu-Dong, Yang, Yang, Zhong, Xiang-Gen, Zhang, Xiang-Hua, Zhang, Yi-Nan, Zheng, Zong-Ping, Zhou, Yu, Tang, Wei, Yang, Yi-Fu, Hu, Li-Hong, Zuo, Jian-Ping
Journal of ethnopharmacology 2008 v.120 no.3 pp. 447-451
Oriental traditional medicine, medicinal plants, macrophages, lipopolysaccharides, anti-inflammatory activity, Annonaceae, immunosuppressive agents, in vitro studies, rheumatoid arthritis, chemical constituents of plants, tumor necrosis factor-alpha, interleukin-6, gene expression, prostaglandin synthase, nitric oxide synthase, cytokines, China
Aim of the study: Fissistigma oldhamii (Hemsl.) Merr, a traditional Chinese herb medicine, is used for treating rheumatoid arthritis in China. In our previous study, an effective compound, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl) ethyl] propenamide (Z23), from this herb has showed potent immunosuppressive effects both in vitro and in vivo. However, its anti-inflammatory effect and mechanism is still need to explore. Materials and methods: We examined the in vitro effects of Z23 on the production of nitric oxide (NO), prostaglandin E2 (PGE2) and cytokines by lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Results: Z23 significantly decreased the production of PGE2, NO, tumour necrosis factor alpha (TNFalpha) and IL6 production. Inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX2) gene expression were also significantly reduced. Conclusions: These results demonstrated that Z23 exerted an anti-inflammatory effect through modulating the synthesis of several mediators and cytokines involved in the inflammatory process. This study provided evidence to understand the therapeutic effects of Fissistigma oldhamii (Hemsl.) Merr and indicated that Z23 has the potential for treatment of various inflammatory diseases where the overproduction of NO, PGE2 and inflammatory cytokines has been shown to play a role, e.g. rheumatoid arthritis.