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Purple acid phosphatase in the walls of tobacco cells

Author:
Kaida, Rumi, Hayashi, Takahisa, Kaneko, Takako S.
Source:
Phytochemistry 2008 v.69 no.14 pp. 2546-2551
ISSN:
0031-9422
Subject:
Nicotiana tabacum, tobacco, cell walls, acid phosphatase, physiological transport, enzyme activity, substrate specificity
Abstract:
Purple acid phosphatase isolated from the walls of tobacco cells appears to be a 220 kDa homotetramer composed of 60 kDa subunits, which is purple in color and which contains iron as its only metal ion. Although the phosphatase did not require dithiothreitol for activity and was not inhibited by phenylarsine oxide, the enzyme showed a higher catalytic efficiency (k(cat)/K(m)) for phosphotyrosine-containing peptides than for other substrates including p-nitrophenyl-phosphate and ATP. The phosphatase formed as a 120 kDa dimer in the cytoplasm and as a 220 kDa tetramer in the walls, where Brefeldin A blocked its secretion during wall regeneration. According to our double-immunofluorescence labeling results, the enzyme might be translocated through the Golgi apparatus to the walls at the interphase and to the cell plate during cytokinesis.
Agid:
744220