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Antioxidative and hepatoprotective effect of CGX, an herbal medicine, against toxic acute injury in mice

Hu, Xiao-Ping, Shin, Jang-Woo, Wang, Jing-Hua, Cho, Jung-Hyo, Son, Jin-Young, Cho, Chong-Kwan, Son, Chang-Gue
Journal of ethnopharmacology 2008 v.120 no.1 pp. 51-55
herbal medicines, medicinal plants, antioxidant activity, medicinal properties, hepatoprotective effect, mice, animal models, liver diseases, dosage, dose response, alanine transaminase, aspartate transaminase, lactate dehydrogenase, liver function, malondialdehyde, blood chemistry, catalase, glutathione, superoxide dismutase, nitric oxide synthase, tumor necrosis factor-alpha
Aim: CGX, a modified traditional Chinese herbal drug whose name means “liver cleaning,” is used to treat various liver disorders. This study investigated the protective effects of CGX and its mechanisms. Material and methods: After pretreating ICR mice twice daily with CGX (po, 50 or 100mg/kg) or distilled water for three consecutive days, acute liver injury was induced by a single injection of CCl₄ (ip, 10mL/kg of 0.2% in olive oil) (n =8 per group). Results: Pretreatment with CGX significantly attenuated the elevation in biochemical parameters, such as alanine transaminase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) in serum, and the malondialdehyde concentrations in liver tissue. Pretreatment with CGX significantly restored the reduction of catalase activity and glutathione (GSH) content, but not superoxide dismutase (SOD) activity, and it inhibited the CCl₄-induced high expression of iNOS and TNF-α in hepatic tissue. Conclusion: This study showed that CGX has hepatoprotective effects against free radical-induced acute injury via primarily antioxidative properties.