A novel l-amino acid ligase from bacillus licheniformis
- Journal of bioscience and bioengineering 2008 v.106 no.3 pp. 313-315
- Bacillus licheniformis
- l-Amino acid α-ligase (EC 22.214.171.124) catalyzed formation of α-peptide bond in unprotected l-amino acids in an ATP-dependent manner. BL00235 gene in Bacillus licheniformis NBRC12200 coded as a new l-amino acid ligase. BL00235 substrate specificity was strict; only methionine or leucine was acceptable as dipeptide N-terminal residues.