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MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull

Author:
Saaby, Lasse, Rasmussen, Hasse B., Jäger, Anna K.
Source:
Journal of ethnopharmacology 2009 v.121 no.1 pp. 178-181
ISSN:
0378-8741
Subject:
quercetin, Calluna vulgaris, chemical constituents of plants, traditional medicine, plant extracts, medicinal plants, medicinal properties, enzyme inhibition, enzyme inhibitors, inhibitory concentration 50, amine oxidase (flavin-containing), Denmark
Abstract:
Aim of the study: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. Materials and methods: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and ¹H NMR. Results: The IC₅₀-value for MAO-A inhibition by quercetin was 18±0.2μM in an assay where the IC₅₀-value for MAO-A inhibition by clorgylin was 0.2±0.02μM. Conclusion: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.
Agid:
745312