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First Report of Ranunculus mild mosaic virus on Ranunculus asiaticus in Yunnan Province, China

Wang, J.H., Zhao, S., Yang, X.M.
Plant disease 2008 v.92 no.11 pp. 1585
Ranunculus asiaticus, Potyvirus, plant viruses, plant diseases and disorders, disease outbreaks, new geographic records, disease diagnosis, pathogen identification, signs and symptoms (plants), enzyme-linked immunosorbent assay, nucleotide sequences, reverse transcriptase polymerase chain reaction, microbial genetics, China
In June 2007, a new viral disease occurred in commercial fields of Ranunculus asiaticus in the Yunnan Province of China. Infected plants exhibited mosaic symptoms and growth abnormalities. Viral disease incidence for this ornamental crop host in the Yunnan Province was estimated to range from 10 to 20%. Electron microscopic examination of negatively stained leaf-dip preparations from symptomatic plants identified long, flexuous linear particles (approximately 800 nm). The samples were tested using indirect antigen-coated plate (ACP)-ELISA. ACP-ELISA results showed that the leaf samples from symptomatic plants reacted positively to the potyvirus group antibody (Agdia Inc., Eklhart, IN). Total nucleic acid extracted from symptomatic plants was tested using reverse transcription (RT)-PCR with primers (S 5'-GGNAAAAYAGYGGNCARCC-3'; M4: 5'-GTTTTCCCAGTCACGAC-3' [N = A, G, C, or T; Y = C or T; and R = A or G]) designed to amplify the 3' terminal region of genomic RNA of the genus Potyvirus (1). RT-PCR produced a 1,650-bp amplification product that was cloned and sequenced (GenBank Accession No. EU684747). The sequenced portion showed 90 and 99% identity with the Ranunculus mild mosaic virus (RMMV) isolates (GenBank Accession Nos. DQ152191 and EF445546) from Italy and Israel, respectively (2). To our knowledge, this is the first report of RMMV in China. Infection from this virus may cause losses for cut-flower production of Ranunculus asiaticu and it is also a potential threat for international trade of Ranunculus germplasm.