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The putative penicillin-binding proteins 1 and 2 are important for viability, growth and cell morphology of Brucella melitensis

Author:
Bandara, Aloka B., Schurig, Gerhardt G., Sriranganathan, Nammalwar, Prasad, Rajeev, Boyle, Stephen M.
Source:
Veterinary microbiology 2009 v.133 no.4 pp. 387-393
ISSN:
0378-1135
Subject:
Brucella melitensis, antibiotic resistance, penicillins, cell biology, cell structures, microbial growth, viability, pathogenicity, microbial genetics, genes, enzymes, binding proteins, cell walls, polysaccharides, strain differences, brucellosis
Abstract:
The penicillin-binding proteins (PBPs) are enzymes that regulate the assembly of the peptidoglycan layer of the bacterial cell wall. The genome of Brucella melitensis strain 16M possesses seven pbp genes: three in pbp-1 family (designated as 1A, 1B, and 1C); one in pbp-2 family; and three in pbp-6 family (designated as 6A, 6B, and 6C). We investigated the importance of pbp-1 and pbp-2 genes to viability, cell morphology and infectivity of B. melitensis. A recombinant B. melitensis strain (designated 16MΔpbp1C) was generated by disrupting the pbp-1C of strain 16M by allelic exchange. This strain produced nearly 20% smaller colonies on trypticase soy agar plates, and grew slower in trypticase soy broth compared to the strain 16M. Electron microscopy revealed that strain 16M exhibited native cocco-bacillus morphology, while 16MΔpbp1C possessed a spherical morphology. Strain 16MΔpbp1C did not differ from strain 16M in terms of recovery from infected mouse macrophage cell line J774.1, or recovery from spleens of infected BALB/c mice, suggesting that pbp-1C is dispensable for intracellular persistence of B. melitensis. Expression of mRNA of fixR, the gene downstream of pbp-1C was similar between the strains 16M and 16MΔpbp1C suggesting that disruption of pbp-1C did not induce any polar effects. Multiple attempts to mutate pbp-1A, pbp-1B, or pbp-2 genes failed, most probably because these genes are indispensable for viability of B. melitensis. Our findings suggest that pbp-1C regulates in vitro growth and cell morphology, whereas pbp-1A, pbp-1B, and pbp-2 are essential for viability of B. melitensis.
Agid:
745811