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Pharmacological properties of the extract and some isolated compounds of Clausena lansium stem bark: Anti-trichomonal, antidiabetic, anti-inflammatory, hepatoprotective and antioxidant effects

Adebajo, A.C., Iwalewa, E.O., Obuotor, E.M., Ibikunle, G.F., Omisore, N.O., Adewunmi, C.O., Obaparusi, O.O., Klaes, M., Adetogun, G.E., Schmidt, T.J., Verspohl, E.J.
Journal of ethnopharmacology 2009 v.122 no.1 pp. 10-19
plant extracts, bark, medicinal plants, medicinal properties, traditional medicine, Clausena lansium, glycemic effect, anti-inflammatory activity, hepatoprotective effect, antioxidant activity, Trichomonas, antiprotozoal agents, malaria, hepatotoxicity, rats, animal disease models, methanol, methylene chloride, insulin, enzyme activity, bronchitis, hepatitis
Ethnopharmacological Relevance: Clausena lansium (Fool's Curry Leaf) is used for various ethnomedical conditions in some countries, including bronchitis, malaria, viral hepatitis, acute and chronic gastro-intestinal inflammation, and as a spicy substitute of the popular Curry leaf tree (Murraya koenigii). Aim of the study: This study was to evaluate the ethnomedical uses of the stem bark in inflammatory conditions, hepatotoxicity and to determine the anti-diabetic and anti-trichomonal properties of the plant. Materials and Method: Anti-trichomonal, in vivo and in vitro antidiabetic and insulin stimulating, anti-inflammatory, hepatoprotective and anti-oxidant activities using Trichomonas gallinae, glucose loaded rats and in vitro insulin secreting cell line (INS-1 cell), carrageenin-induced rat paw oedema, CCl₄-induced hepatotoxicity and DPPH scavenging ability methods respectively for the extracts and some isolates were determined. Results: A dichloromethane extract was superior over methanolic extract with respect to an anti-trichomonal activity which was measured after 24 and 48h. The isolated compounds imperatorin and 3-formylcarbazole had the main anti-trichomonal activity (LC₅₀s of 6.0, 3.0 and 3.6, 9.7μg/mL after 24 and 48h, respectively). Methanolic extract (100mg/kg) induced maximum and significant (p <0.05) anti-hyperglycaemic activity of 15.8% at 30min and a 38.5% increase in plasma insulin at 60min, compared to control. The increase in plasma insulin after 60min, compared to 0min, was 62.0% (p <0.05). The significant 174.6% increase of insulin release from INS-1 cells (in vitro) at 0.1mg/ml indicates that it mediates its antidiabetic action mainly by stimulating insulin release. Imperatorin and chalepin were the major active constituents increasing in vitro insulin release to 170.3 and 137.9%, respectively. 100mg/kg of the methanolic extract produced an anti-inflammatory activity after 4h. A sedative effect was not observed. 100 and 200mg/kg of methanolic extract administered i.p., reduced CCl4-induced hepatotoxicity firstly by 5.3 and 8.4% reduction in phenobarbitone-sleeping time respectively, secondly by reversing the reduction in serum liver proteins by 7.0-8.8%, serum AST, ALT and ALP activities by 27.7-107.9% and thirdly by diminishing increased values of plasma AST, ALT and ALP activities by 13.2-83.8%. The extract exhibited antioxidant activities. Conclusion: The hepatoprotective activity of C. lansium is partly due to its anti-oxidant and anti-inflammatory properties and confirms its folkloric use in the treatment of gastro-intestinal inflammation, bronchitis and hepatitis. In addition the use of C. lansium stem bark would be useful in diabetes and trichomoniasis.