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Anti-inflammatory activity of a water extract of Acorus calamus L. leaves on keratinocyte HaCaT cells

Kim, Hyeri, Han, Tae-Ho, Lee, Seong-Gene
Journal of ethnopharmacology 2009 v.122 no.1 pp. 149-156
plant extracts, leaves, Acorus calamus, keratinocytes, human cell lines, medicinal plants, anti-inflammatory activity, reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, fluorescent antibody technique, immunoblotting, cytokines, interleukin-8, interleukin-6, gene expression, skin diseases
Ethnopharmacological relevance: Acorus calamus L., sweet flag, is a well-known medicinal plant that grows worldwide wildly along swamps, rivers, and lakes. Aim of the study: The aim of this study was to evaluate the anti-inflammatory activity of Acorus calamus leaf (ACL) extract and to explore its mechanism of action on human keratinocyte HaCaT cells. Materials and methods: HaCaT cells treated with polyinosinic:polycytidylic acid (polyI:C) and peptidoglycan (PGN) induced the inflammatory reactions. The anti-inflammatory activities of ACL were investigated using RT-PCR, ELISA assay, immunoblotting, and immunofluorescence staining. Results: HaCaT cells induced the pro-inflammatory cytokines, interleukin-8 (IL-8) and/or interleukin-6 (IL-6) expressions after treatment with polyI:C or PGN. ACL inhibited the expression of IL-8 and IL-6 RNA and protein levels, and attenuated the activation of NF-κB and IRF3 after polyI:C treatment. ACL also inhibited expression of IL-8 and activation of NF-κB following PGN induction. Conclusions: These results suggest that ACL inhibits the production of pro-inflammatory cytokines through multiple mechanisms and may be a novel and effective anti-inflammatory agent for the treatment of skin diseases.