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Advantage of using PSIRB over PSRB and IRB to improve plant health of tomato

Author:
Hariprasad, P., Navya, H.M., Chandra Nayaka, S., Niranjana, S.R.
Source:
Biological control 2009 v.50 no.3 pp. 307-316
ISSN:
1049-9644
Subject:
seedling growth, plant growth-promoting rhizobacteria, plant pathogenic fungi, tomatoes, interspecific variation, fungal antagonists, systemic acquired resistance, vegetable crops, Fusarium wilt, phytic acid, strain differences, disease control, rhizosphere bacteria, Fusarium, Solanum lycopersicum var. lycopersicum, seed germination, biological control, disease incidence
Abstract:
Twenty-one isolates of phosphate solubilizing-indole acetic acid producing rhizobacteria (PSIRB), 20 isolates of phosphate solubilizing rhizobacteria (PSRB) and 42 isolates of indole acetic acid producing rhizobacteria (IRB) were isolated from 49 rhizospheric soil samples of tomato (Lycopersicon esculentum Mill.) collected from tomato growing regions of Karnataka. A method combining Pikovskaya's and Bric's technique was developed to isolate PSRIB, PSRB and IRB's. The selected isolates were further analyzed for their ability to solubilize calcium phytate. Based on the root colonization assays and the abilities of bacterial isolates to increase the seed germination and seedling vigor under laboratory conditions, five isolates were selected from each group for further studies. Under greenhouse conditions, all the selected rhizobacteria isolates significantly increased root length, shoot length, fresh weight, dry weight and total phosphorus content of 30-day-old-seedlings with respect to control. Isolate PSIRB1 and IRB36 significantly reduced the Fusarium wilt incidence over other isolates of same and other group, and the control. On the basis of results from laboratory and greenhouse studies, one bacterial isolate from each group was selected for plant growth and yield analysis studies. Isolate PSIRB2 showed increased plant height, fresh weight, number of fruits per plant and average weight of fruit over PSRB9, IRB36 and untreated controls. Studies on the nature of protection offered by these bacterial isolates following split-root technique revealed that the isolates PSIRB2 and PSRB9 had the ability to induce systemic resistance. One isolate, IRB36 appeared to protect the tomato seedlings through direct antagonism.
Agid:
760663