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Preparation of Free-Standing Films from Kafirin Protein Microparticles: Mechanism of Formation and Functional Properties

Author:
Taylor, Janet, Taylor, John R.N., Belton, Peter S., Minnaar, Amanda
Source:
Journal of agricultural and food chemistry 2009 v.57 no.15 pp. 6729-6735
ISSN:
0021-8561
Subject:
films (materials), kafirins, functional properties, plasticizers, acetic acid, protein aggregates, biopolymers, Sorghum bicolor, protein content, acidity
Abstract:
A method of preparing free-standing films using kafirin microparticles made by phase separation from acetic acid is described. Film preparation involved the suspension of the microparticles in acetic acid solution containing plasticizer. On evaporation of the acetic acid, a complete, smooth, flexible, transparent film was formed. A minimum concentration of acid was required to form a cohesive film relative to the concentration of kafirin. This was approximately 10.8:1, percent acetic acid to percent kafirin. Film formation appears to be by controlled aggregation of kafirin microparticles, followed by dissolution of the microparticles in acetic acid and drying into a cohesive film. The functional properties of microparticle films were generally superior to films cast directly from a solution of kafirin, at the same protein content. Kafirin microparticle films were very thin (<15 μm), relatively strong but not very extensible, with better water barrier properties and lower protein digestibility than conventionally cast kafirin films.
Agid:
762387