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Transplacental toxoplasmosis in a wild southern sea otter (Enhydra lutris nereis)

Author:
Miller, Melissa, Conrad, Patricia, James, E.R., Packham, Andrea, Toy-Choutka, Sharon, Murray, Michael J., Jessup, David, Grigg, Michael
Source:
Veterinary parasitology 2008 v.153 no.1-2 pp. 12-18
ISSN:
0304-4017
Subject:
transplacental transmission, maternal-fetal transfer, toxoplasmosis, wild animals, wildlife diseases, Toxoplasma gondii, DNA, nucleotide sequences, Enhydra lutris, case studies, necropsy, congenital abnormalities, histopathology, brain
Abstract:
In September 2004, a neonatal sea otter pup was found alive on the beach in northern Monterey Bay, CA. Efforts to locate the mother were unsuccessful. Due to a poor prognosis for successful rehabilitation, the pup was euthanized. Postmortem examination revealed emaciation, systemic lymphadenopathy and a malformation of the left cerebral temporal lobe. On histopathology, free tachyzoites and tissue cysts compatible with Toxoplasma gondii were observed in the brain, heart, thymus, liver, lymph nodes and peri-umbilical adipose. The presence of T. gondii within host tissues was associated with lymphoplasmacytic inflammation and tissue necrosis. Immunofluorescent antibody tests using postmortem serum were positive for anti-T. gondii IgM and IgG (at 1:320 and 1:1280 serum dilution, respectively), but were negative for IgG directed against Sarcocystis neurona and Neospora caninum (<1:40 each). Brain immunohistochemistry revealed positive staining for tachyzoites and tissue cysts using antiserum raised to T. gondii, but not S. neurona or N. caninum. T. gondii parasite DNA was obtained from extracts of brain and muscle by PCR amplification using the diagnostic B1 locus. Restriction enzyme digestion followed by gel electrophoresis and DNA sequencing confirmed the presence of Type X T. gondii, the strain identified in the majority of southern sea otter infections.
Agid:
763343