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Effects of four tropical tanniniferous plant extracts on the inhibition of larval migration and the exsheathment process of Trichostrongylus colubriformis infective stage

Author:
Alonso-Díaz, M.A., Torres-Acosta, J.F.J., Sandoval-Castro, C.A., Capetillo-Leal, C., Brunet, S., Hoste, H.
Source:
Veterinary parasitology 2008 v.153 no.1-2 pp. 187-192
ISSN:
0304-4017
Subject:
tropical and subtropical horticulture, Trichostrongylus colubriformis, Piscidia, Leucaena leucocephala, plant extracts, larval development, disease control, animal parasitic nematodes, tannins, Acacia pennatula, nematode larvae, in vitro studies, ecdysis
Abstract:
The anthelmintic (AH) effect of Acacia pennatula, Leucaena leucocephala, Lisyloma latisiliquum and Piscidia piscipula was evaluated in the infective larvae (L₃) of Trichostrongylus colubriformis. Different concentrations of lyophilized extracts were tested using the larval migration inhibition (LMI) test. An inhibitor of tannins (the polyvinyl polypyrrolidone [PVPP]) was used to verify whether these compounds were responsible for the AH effects. Then, the effect of extracts on larval exsheathment was examined by observing the exsheathment process at 10-min intervals for 70min. The LMI test showed a dose-dependant AH effect for A. pennatula, L. leucocephala and L. latisiliquum (P <0.01), but not for P. piscipula. The restoration of L₃ migration to values similar to those of controls after the addition of PVPP, indicates that tannins are involved in AH effects. Trichostrongylus colubriformis exsheathment was partially or totally blocked by the four plants extracts. Tropical tanniniferous plants evaluated in the current study may have potential as AH for the control of T. colubriformis if in vivo investigations indicate useful effects.
Agid:
763355