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Species identification of Trichinella isolates from China

Author:
Fu, B.Q., Liu, M.Y., Yao, C.Y., Li, W.H., Li, Y.G., Wang, Y.H., Wu, X.P., Zhang, D.L., Cai, X.P., Blaga, R., Boireau, P.
Source:
Veterinary parasitology 2009 v.159 no.3-4 pp. 214-217
ISSN:
0304-4017
Subject:
interspecific variation, mitochondrial DNA, ribosomal DNA, polymerase chain reaction, Trichinella spiralis, animal parasitic nematodes, nucleotide sequences, genetic variation, pest identification, China
Abstract:
Two species of Trichinella were identified from China by means of PCR amplification of the mitochondrial small subunit ribosomal DNA and the expansion segment V region of the ribosomal DNA. Seven isolates originating from domestic pig and one isolate originating from dog showed identical DNA banding pattern to Trichinella spiralis, and two isolates from dog showed DNA banding pattern identical to Trichinella nativa. Sequence analysis of the 5S rDNA inter-gene spacer region from the ten Trichinella isolates confirmed the existence of only two Trichinella species and revealed the inner species genetic variation within T. spiralis and T. nativa.
Agid:
763510