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Molecular identification and genetic analysis for 24 turf-type Cynodon cultivars by Sequence-Related Amplified Polymorphism markers

Wang, Zhiyong, Yuan, Xuejun, Zheng, Yiqi, Liu, Jianxiu
Scientia horticulturae 2009 v.122 no.3 pp. 461-467
Cynodon, turf grasses, molecular genetics, cultivars, genetic variation, phylogeny, genetic relationships, genetic markers, nucleotide sequences, sequence alignment, gene amplification, genetic code, genetic distance, genotype, germplasm screening, cultivar identification, geographical variation, China, Australia, United States
Bermudagrass (Cynodon ssp.) germplasm is genetically diverse and widely distributed in the world. The study was conducted to identify and assess the molecular variation and relationship among 24 cultivars developed in China, Australia and the USA. Sequence-Related Amplified Polymorphism (SRAP) was applied to cultivars identification in this study for the first time. Thirty of the 90 SRAP primer combinations generated a total of 274 clearly bands encompassing 249 (91%) polymorphic. Each bermudagrass cultivar has its unique binary code and can be distinguished from the others. Three distinct clusters were obtained by unweighted pair-group method with arithmetic averages based on the polymorphic markers. Coefficients of genetic distance among the genotypes ranged from 0.57 to 0.97. The results demonstrated that SRAP marker is a stable molecular marker technique for the identification of bermudagrass cultivars and their genetic relationships.