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A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Liu, Li, Wang, Aili, Appels, Rudi, Ma, Junhong, Xia, Xianchun, Lan, Ping, He, Zhonghu, Bekes, Frank, Yan, Yueming, Ma, Wujun
Journal of cereal science 2009 v.50 no.2 pp. 295-301
Triticum aestivum, wheat, glutenins, molecular weight, genotype, alleles, mass spectrometry, polyacrylamide gel electrophoresis
High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki.