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Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)

Author:
Xu, Z., Chang, M.X., Qi, Z.T., Huang, B., Nie, P.
Source:
Veterinary immunology and immunopathology 2009 v.131 no.3-4 pp. 268-272
ISSN:
0165-2427
Subject:
turtles, Trionychidae, immunoglobulins, binding properties, molecular genetics, complementary DNA, sequence analysis, open reading frames, amino acid sequences, species differences, chickens, phylogeny, reverse transcriptase polymerase chain reaction, molecular cloning, immune response, bacterial infections, Aeromonas hydrophila, transcriptional activation, blood, spleen, kidneys
Abstract:
The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347bp in length and contains an open reading frame of 480bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection.
Agid:
765000