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Structural characterisation and antioxidant properties of polysaccharides from the fruiting bodies of Russula virescens

Sun, Zhong-wei, Zhang, Li-xiang, Zhang, Bin, Niu, Tian-gui
Food chemistry 2010 v.118 no.3 pp. 675-680
antioxidant activity, polysaccharides, glycosidic linkages, reduction, fruiting bodies, chelating agents, fractionation, mushrooms, Russula, free radical scavengers, water solubility
Water-soluble crude polysaccharide named as RVP was obtained from the fresh fruiting bodies of Russula virescens by boiling-water extraction. DEAE-Sepharose CL-6B column chromatography was used for the fractionation of polysaccharide RVP. Two fractions were obtained, namely RVP-1 and RVP-2. RVP-1 and RVP-2 were composed mainly of glucose, with the estimated equivalent dextran molecular weights of 3.1x10⁵ and 4.2x10⁵ Da, respectively. Analysis by Periodate oxidation-Smith degradation indicated that RVP-1 was composed of 68.3% (1[rightward arrow])- or ((1[rightward arrow]6)-glycosidic linkages and 31.7% (1[rightward arrow]3)-glycosidic linkages, and RVP-2 7.9% (1[rightward arrow])- or (1[rightward arrow]6)-glycosidic linkages, 9.6% (1[rightward arrow]2)-glycosidic linkages, 35.7% (1[rightward arrow]4)-glycosidic linkages, and 46.8% (1[rightward arrow]3)-glycosidic linkages. RVP-1 exhibited equivalent inhibiting power for self-oxidation of 1,2,3-phentriol to vitamin C (Vc), a little higher scavenging activity of superoxide radical and hydroxyl radical than Vc. The reducing power of RVP-1 at 20.0mg/ml was 0.77, and RVP-1 was a good chelating agent for ferrous ions. Overall, RVP-1 possessed good antioxidant properties and should be developed as a novel potential antioxidant.