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Catechin-loaded calcium pectinate microparticles reinforced with liposome and hydroxypropylmethylcellulose: Optimization and in vivo antioxidant activity

Lee, Ji-Soo, Kim, Hye Won, Chung, Donghwa, Lee, Hyeon Gyu
Food hydrocolloids 2009 v.23 no.8 pp. 2226-2233
pectins, catechin, microencapsulation, methylcellulose, liposomes (artificial), calcium chloride, chemical concentration, optimization, response surface methodology, application methods, gastric juice, intestines, digestive juices, antioxidant activity, phytochemicals, rats, animal models, human nutrition, in vivo studies
The preparation conditions of Ca-pectinate microparticles reinforced with liposome and hydroxypropylmethylcellulose (HPMC), including CaCl₂ concentration (X₁), HPMC concentration (X₂), and hardening time (X₃), were optimized for the sustained-release of catechin into simulated gastric and intestinal fluids using response surface methodology. The optimized values of X₁, X₂, and X₃ were found to be 5.20%, 0.08%, and 12.63 min, respectively. The microparticles prepared according to the optimized conditions released about 50% of the entrapped catechin into simulated gastric fluid during 24-h incubation period, whereas almost complete catechin release was observed in simulated intestinal fluid after 8-h incubation. Antioxidant activity of catechin in rat plasma was more effectively maintained when the catechin-loaded microparticles were fed, compared to the case of feeding free catechin, indicating that the microparticles can protect catechin molecules in the rat model and release them in a sustained manner.