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Catechin-loaded calcium pectinate microparticles reinforced with liposome and hydroxypropylmethylcellulose: Optimization and in vivo antioxidant activity
- Lee, Ji-Soo, Kim, Hye Won, Chung, Donghwa, Lee, Hyeon Gyu
- Food hydrocolloids 2009 v.23 no.8 pp. 2226-2233
- pectins, catechin, microencapsulation, methylcellulose, liposomes (artificial), calcium chloride, chemical concentration, optimization, response surface methodology, application methods, gastric juice, intestines, digestive juices, antioxidant activity, phytochemicals, rats, animal models, human nutrition, in vivo studies
- The preparation conditions of Ca-pectinate microparticles reinforced with liposome and hydroxypropylmethylcellulose (HPMC), including CaCl₂ concentration (X₁), HPMC concentration (X₂), and hardening time (X₃), were optimized for the sustained-release of catechin into simulated gastric and intestinal fluids using response surface methodology. The optimized values of X₁, X₂, and X₃ were found to be 5.20%, 0.08%, and 12.63 min, respectively. The microparticles prepared according to the optimized conditions released about 50% of the entrapped catechin into simulated gastric fluid during 24-h incubation period, whereas almost complete catechin release was observed in simulated intestinal fluid after 8-h incubation. Antioxidant activity of catechin in rat plasma was more effectively maintained when the catechin-loaded microparticles were fed, compared to the case of feeding free catechin, indicating that the microparticles can protect catechin molecules in the rat model and release them in a sustained manner.