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Comparative Methodologies for Measuring Metabolizable Energy of Various Types of Resistant High Amylose Corn Starch

Tulley, Richard T., Appel, Marko J., Enos, Tanya G., Hegsted, Maren, McCutcheon, Kathleen L., Zhou, Jun, Raggio, Anne M., Jeffcoat, Roger, Birkett, Anne, Martin, Roy J., Keenan, Michael J.
Journal of agricultural and food chemistry 2009 v.57 no.18 pp. 8474–8479
methodology, measurement, metabolizable energy, measuring devices, resistant starch, corn starch, amylose, rats, animal models, experimental diets, in vitro digestion, metabolic studies
Energy values of high amylose corn starches high in resistant starch (RS) were determined in vivo by two different methodologies. In one study, energy values were determined according to growth relative to glucose-based diets in rats fed diets containing RS2, heat-treated RS2 (RS2−HT), RS3, and amylase predigested versions to isolate the RS component. Net metabolizable energy values ranged from 2.68 to 3.06 kcal/g for the RS starches, and 1.91−2.53 kcal/g for the amylase predigested versions. In a second study, rats were fed a diet containing RS2−HT and the metabolizable energy value was determined by bomb calorimetry. The metabolizable energy value was 2.80 kcal/g, consistent with Study 1. Thus, high amylose corn based RS ingredients and their amylase predigested equivalents have energy values approximately 65−78% and 47−62% of available starch (Atwater factor), respectively, according to the RS type (Garcia, T. A.; McCutcheon, K. L.; Francis, A. R.; Keenan, M. J.; O’Neil, C. E.; Martin, R. J.; Hegsted, M.The effects of resistant starch on gastrointestinal organs and fecal output in rats.FASEB J. 2003, 17, A335).