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Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae

Chao Shen, Yimei Cai, Jialing Li, Chengyong He, Zhenghong Zuo
Journal of environmental sciences (China) 2023 v.124 pp. 76-88
Danio rerio, anilinopyrimidine fungicides, aryl hydrocarbon receptors, developmental toxicity, fruit growing, fungi, genes, light intensity, mepanipyrim, opsin, phototransduction, quantitative polymerase chain reaction, transcriptomics, wavelengths, China
Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked escape experiments were used to investigate vision-guided behaviors. Meanwhile, transcriptomic sequencing and real-time quantitative PCR assays were applied to assess the potential mechanisms of mepanipyrim-induced visual developmental toxicity and vision-guided behavioral alteration. Our results showed that mepanipyrim exposure could induce retinal impairment and vision-guided behavioral alteration in larval zebrafish. In addition, the grk1b gene of the phototransduction signaling pathway was found to be a potential aryl hydrocarbon receptor (AhR)-regulated gene. Mepanipyrim-induced visual developmental toxicity was potentially related to the AhR signaling pathway. Furthermore, mepanipyrim-induced behavioral alteration was guided by the visual function, and the effects of mepanipyrim on long and middle wavelength light-sensitive opsins may be the main cause of vision-guided behavioral alteration. Our results provide insights into understanding the relationship between visual development and vision-guided behaviors induced by mepanipyrim exposure.