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Dynamics of plc gene transcription and α-toxin production during growth of Clostridium perfringens strains with contrasting α-toxin production

Author:
Abildgaard, Lone, Schramm, Andreas, Rudi, Knut, Højberg, Ole
Source:
Veterinary microbiology 2009 v.139 no.1-2 pp. 202-206
ISSN:
0378-1135
Subject:
chickens, necrotic enteritis, Clostridium perfringens, pathogenesis, pathotypes, bacterial toxins, biosynthesis, microbial growth, molecular genetics, transcription (genetics), genes, phospholipase C, translation (genetics), strain differences, virulence, gene expression, microbial genetics
Abstract:
The aim of the present study was to investigate transcription dynamics of the α-toxin-encoding plc gene relative to two housekeeping genes (gyrA and rplL) in batch cultures of three Clostridium perfringens strains with low, intermediate, and high levels of α-toxin production, respectively. The plc transcript level was always low in the low α-toxin producing strain. For the two other strains, plc transcription showed an inducible pattern and reached a maximum level in the late exponential growth phase. The transcription levels were however inversely correlated to α-toxin production for the two strains. We propose that this discrepancy is due to differences in plc translation rates between the strains and that strain-specific translational rates therefore must be determined before α-toxin production can be extrapolated from transcript levels in C. perfringens.
Agid:
766577