U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Zn2+ loading as a critical contributor to the circ_0008553-mediated oxidative stress and inflammation in response to PM2.5 exposures

Jingzhou Wang, Jianbo Jia, Dujia Wang, Xiujiao Pan, Haiyan Xiong, Chengjun Li, Yiguo Jiang, Bing Yan
Journal of environmental sciences (China) 2023 v.124 pp. 451-461
Internet, epithelium, humans, inflammation, microRNA, models, oxidative stress, particulates, synergism, China
Inflammation is a major adverse outcome induced by inhaled particulate matter with a diameter of ≤ 2.5 μm (PM₂.₅), and a critical trigger of most PM₂.₅ exposure-associated diseases. However, the key molecular events regulating the PM₂.₅-induced airway inflammation are yet to be elucidated. Considering the critical role of circular RNAs (circRNAs) in regulating inflammation, we predicted 11 circRNAs that may be involved in the PM₂.₅-induced airway inflammation using three previously reported miRNAs through the starBase website. A novel circRNA circ_0008553 was identified to be responsible for the PM₂.₅-activated inflammatory response in human bronchial epithelial cells (16HBE) via inducing oxidative stress. Using a combinatorial model PM₂.₅ library, we found that the synergistic effect of the insoluble core and loaded Zn²⁺ ions at environmentally relevant concentrations was the major contributor to the upregulation of circ_0008553 and subsequent induction of oxidative stress and inflammation in response to PM₂.₅ exposures. Our findings provided new insight into the intervention of PM₂.₅-induced adverse outcomes.