U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Understanding the effects of process parameters in the bioscouring of cotton and their interactions on pectate lyase activity by factorial design analysis

Author:
Bruna Lyra Colombi, Quesli Martins, Cintia Kopsch Imme, Diofer Barboza Da Silva, José Alexandre Borges Valle, Jürgen Andreaus, Manuel José Lis Arias, Rita De Cássia Siqueira Curto Valle
Source:
The journal of the Textile Institute 2022 v.113 no.5 pp. 857-868
ISSN:
1754-2340
Subject:
bioscouring, cotton fabric, experimental design, hydrophobicity, industrial applications, nonionic surfactants, pH, pectate lyase, polygalacturonic acid, temperature, wettability
Abstract:
Pectinases can be used to remove impurities and hydrophobic material from cotton fibers by the degradation of pectic substances. The biochemical characterization and the evaluation of process parameters that influence the enzyme’s activity are mandatory to optimize the industrial application. In the present study, a factorial experimental design was conducted to evaluate the activity of a commercial pectate lyase at different reaction conditions of temperature, pH, and nonionic surfactant concentration, aiming its application in bioscouring of textiles. The main effects of all three factors were found to be statistically significant. The second-order interactions between temperature and pH, and pH and surfactant concentration, were also important. Maximum activity on polygalacturonic acid was achieved at 60 °C, pH 9, and without surfactant. These results provided support for the subsequent bioscouring assays, resulting in a high level of cleanliness and good wettability of knitted cotton fabric at the lowest enzyme concentration (1 g/l), and could make textile processing more sustainable.
Agid:
7762884