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Genetic diversity in Acorus calamus L. as revealed by RAPD markers and its relationship with β-asarone content and ploidy level

Ahlawat, Anita, Katoch, Meenu, Ram, Gandhi, Ahuja, Ashok
Scientia horticulturae 2010 v.124 no.2 pp. 294-297
Acorus calamus, medicinal plants, medicinal properties, genetic markers, genetic variation, phytochemicals, polyploidy, random amplified polymorphic DNA technique, genetic polymorphism, genetic distance, phylogeny, triploidy, tetraploidy, provenance
β-Asarone content in Acorus calamus is a paramount issue because it limits the usage of plant for medicinal purpose. In the present study A. calamus L. accessions based on RAPD marker, ploidy level and β-asarone content were characterized and correlated on the basis of β-asarone content/ploidy level. Of the 40 random primers used, 6 primers generated polymorphism. Genetic relatedness among accessions evaluated by a similarity matrix based on Dice's coefficient ranged from 0.72 to 0.97. A phenetic dendrogram based on UPGMA analysis grouped accessions into two clusters. A. calamus L. accessions were found to be triploid and tetraploid and their β-asarone content was found in two ranges 6.92-8.0% and 73-88%. The study clustered the accessions as per their ploidy level, β-asarone content and geographical locations. This study would have extensive application in quality control of raw materials.